Journal: The Journal of Antibiotics

Article Title: Potent Rifampicin derivatives can clear MRSA infections at single low doses when concomitantly dosed with Vancomycin

doi: 10.1038/s41429-023-00663-6

Figure Lengend Snippet: Cellular activity of rifampicin-based antibiotics

Article Snippet: For the assay, S. aureus NRS384 (BEI Resources), S. typhimurium AR-0031 (CDC & FDA Antibiotic Resistance Isolate Bank, Atlanta (GA): CDC 2018) (and S. typhimurium ATCC 14028 (American Type Culture Collection) were grown in Tryptic Soy Broth (TSB, Teknova) overnight, then sub-cultured 1:50 in fresh TSB and grown for an additional 2 h. The cultures were then pelleted via centrifugation and washed twice in PBS (Gibco).

Techniques: Activity Assay

Journal: The Journal of Antibiotics

Article Title: Potent Rifampicin derivatives can clear MRSA infections at single low doses when concomitantly dosed with Vancomycin

doi: 10.1038/s41429-023-00663-6

Figure Lengend Snippet: Mice infected with S. aureus MRSA strain NRS384 then treated with vancomycin (110 mg twice daily) and rifampicin. *** p < 0.001, * p < 0.05, ns = not significant ( t -test). Figure adapted from PCT WO 2022204499

Article Snippet: For the assay, S. aureus NRS384 (BEI Resources), S. typhimurium AR-0031 (CDC & FDA Antibiotic Resistance Isolate Bank, Atlanta (GA): CDC 2018) (and S. typhimurium ATCC 14028 (American Type Culture Collection) were grown in Tryptic Soy Broth (TSB, Teknova) overnight, then sub-cultured 1:50 in fresh TSB and grown for an additional 2 h. The cultures were then pelleted via centrifugation and washed twice in PBS (Gibco).

Techniques: Infection

Journal: The Journal of Antibiotics

Article Title: Potent Rifampicin derivatives can clear MRSA infections at single low doses when concomitantly dosed with Vancomycin

doi: 10.1038/s41429-023-00663-6

Figure Lengend Snippet: Mice infected with S. aureus MRSA strain NRS384 then treated with vancomycin (110 mg twice daily) and compounds 4 and 5 . p < 0.0001 (two-way ANOVA). Figure adapted from PCT WO 2022204499

Article Snippet: For the assay, S. aureus NRS384 (BEI Resources), S. typhimurium AR-0031 (CDC & FDA Antibiotic Resistance Isolate Bank, Atlanta (GA): CDC 2018) (and S. typhimurium ATCC 14028 (American Type Culture Collection) were grown in Tryptic Soy Broth (TSB, Teknova) overnight, then sub-cultured 1:50 in fresh TSB and grown for an additional 2 h. The cultures were then pelleted via centrifugation and washed twice in PBS (Gibco).

Techniques: Infection

Journal: The Journal of Antibiotics

Article Title: Potent Rifampicin derivatives can clear MRSA infections at single low doses when concomitantly dosed with Vancomycin

doi: 10.1038/s41429-023-00663-6

Figure Lengend Snippet: Mice infected with S. aureus MRSA strain NRS384 and treated with or without vancomycin (110 mg twice daily) and compound 5 . ns = not significant, ** p < 0.005 (two-way ANOVA). Figure adapted from PCT WO 2022204499

Article Snippet: For the assay, S. aureus NRS384 (BEI Resources), S. typhimurium AR-0031 (CDC & FDA Antibiotic Resistance Isolate Bank, Atlanta (GA): CDC 2018) (and S. typhimurium ATCC 14028 (American Type Culture Collection) were grown in Tryptic Soy Broth (TSB, Teknova) overnight, then sub-cultured 1:50 in fresh TSB and grown for an additional 2 h. The cultures were then pelleted via centrifugation and washed twice in PBS (Gibco).

Techniques: Infection

Journal: Frontiers in Microbiology

Article Title: Comparative Analysis of Virulence and Toxin Expression of Vancomycin-Intermediate and Vancomycin-Sensitive Staphylococcus aureus Strains

doi: 10.3389/fmicb.2020.596942

Figure Lengend Snippet: The Staphylococcus aureus strains used in this study.

Article Snippet: The clinical S. aureus strain SA0534 and laboratory-induced vancomycin resistance strains (SA0534-V8 and SA0534-V16) were obtained from Yuanyuan Dai at The Affiliated Provincial Hospital of Anhui Medical University ( ).

Techniques:

Journal: Frontiers in Microbiology

Article Title: Comparative Analysis of Virulence and Toxin Expression of Vancomycin-Intermediate and Vancomycin-Sensitive Staphylococcus aureus Strains

doi: 10.3389/fmicb.2020.596942

Figure Lengend Snippet: Results of mouse skin infection model experiments. (A) Is the photo of the comparison of abscess area of BALB/C-nude mice infected with VSSA and VISA strains. (B) Is line chart of the abscess area. Each strain injected six mice. The VS- indicates that this strain belongs to VSSA. The VI- indicates that the strain belongs to VISA. The SA0534-V8 and SA0534-V16 is VISA strains while SA0534 is a VSSA strain. Two-way analysis of variance was used to compare data for multiple groups.

Article Snippet: The clinical S. aureus strain SA0534 and laboratory-induced vancomycin resistance strains (SA0534-V8 and SA0534-V16) were obtained from Yuanyuan Dai at The Affiliated Provincial Hospital of Anhui Medical University ( ).

Techniques: Infection, Comparison, Injection

Journal: Frontiers in Microbiology

Article Title: Comparative Analysis of Virulence and Toxin Expression of Vancomycin-Intermediate and Vancomycin-Sensitive Staphylococcus aureus Strains

doi: 10.3389/fmicb.2020.596942

Figure Lengend Snippet: Comparison of survival rates of VSSA and VISA infected with Galleria mellonella larvae. G . mellonella larvae were inoculated with 10 μl each pair at doses ranging from 1 × 10 4 and incubated at 37°C; the viability was assessed over 40 h. The VS- indicates that this strain belongs to VSSA. The VI- indicates that the strain belongs to VISA. The SA0534-V8 and SA0534-V16 is VISA strains while SA0534 is a VSSA strain. (A) Is the survival rates of VS-2393 and VI-2562 infected with G. mellonella larvae. (B) Is the survival rates of VS-1201 and VI-1130 infected with G. mellonella larvae. (C) Is the survival rates of SA0534, SA0534-V8, and SA0534-V16 infected with G. mellonella larvae.

Article Snippet: The clinical S. aureus strain SA0534 and laboratory-induced vancomycin resistance strains (SA0534-V8 and SA0534-V16) were obtained from Yuanyuan Dai at The Affiliated Provincial Hospital of Anhui Medical University ( ).

Techniques: Comparison, Infection, Incubation

Journal: Frontiers in Microbiology

Article Title: Comparative Analysis of Virulence and Toxin Expression of Vancomycin-Intermediate and Vancomycin-Sensitive Staphylococcus aureus Strains

doi: 10.3389/fmicb.2020.596942

Figure Lengend Snippet: The comparison of nasal colonization and biofilm formation ability of VISA and VSSA strains. Values are means ± SD (three repeated different experiments). * P < 0.05. The VS- indicates that this strain belongs to VSSA. The VI- indicates that the strain belongs to VISA. The SA0534-V8 and SA0534-V16 is VISA strains while SA0534 is a VSSA strain. (A) Is the nasal colonization ability of VISA strains in mice ( n = 5) compared with that of VISA strains. (B) Is the biofilm formation ability of VISA and VSSA strains.

Article Snippet: The clinical S. aureus strain SA0534 and laboratory-induced vancomycin resistance strains (SA0534-V8 and SA0534-V16) were obtained from Yuanyuan Dai at The Affiliated Provincial Hospital of Anhui Medical University ( ).

Techniques: Comparison

Journal: Frontiers in Microbiology

Article Title: Comparative Analysis of Virulence and Toxin Expression of Vancomycin-Intermediate and Vancomycin-Sensitive Staphylococcus aureus Strains

doi: 10.3389/fmicb.2020.596942

Figure Lengend Snippet: α-toxin activity and production between VSSA and VISA strains. Spectrophotometer was used to calculated the absorbance (A600 nm) of each sample. The value of complete hemolysis group (positive control) was set as 100%. ELISA was used to semi-quantify the concentration of α-toxin. Supernatant without antibody was used as a negative control. Less than twice the value of the negative control was defined as the cut-off value. Values are means ± SD (three repeated different experiments). * P < 0.05, ** P < 0.01. The VS- indicates that this strain belongs to VSSA. The VI- indicates that the strain belongs to VISA. The SA0534-V8 and SA0534-V16 is VISA strains while SA0534 is a VSSA strain. (A) Is the α-toxin activity and production between VS-2393 and VI-2562. (B) Is the α-toxin activity and production between VS-1201 and VI-1130. (C) Is the α-toxin activity and production among SA0534, SA0534-V8, and SA0534-V16.

Article Snippet: The clinical S. aureus strain SA0534 and laboratory-induced vancomycin resistance strains (SA0534-V8 and SA0534-V16) were obtained from Yuanyuan Dai at The Affiliated Provincial Hospital of Anhui Medical University ( ).

Techniques: Activity Assay, Spectrophotometry, Positive Control, Enzyme-linked Immunosorbent Assay, Concentration Assay, Negative Control

Journal: Frontiers in Microbiology

Article Title: Comparative Analysis of Virulence and Toxin Expression of Vancomycin-Intermediate and Vancomycin-Sensitive Staphylococcus aureus Strains

doi: 10.3389/fmicb.2020.596942

Figure Lengend Snippet: Intracellular cytotoxicity of VSSA and VISA strains in human neutrophils. We performed the cytotoxicity assay, following controls. (1) Exp. value: the absorbance value of each experimental sample. (2) Low control: the absorbance value of culture medium background. (3) High control: the absorbance value of the positive control (maximum LDH release). Values are means ± SD (three repeated different experiments). * P < 0.05, ** P < 0.01. The VS- indicates that this strain belongs to VSSA. The VI- indicates that the strain belongs to VISA. The SA0534-V8 and SA0534-V16 is VISA strains while SA0534 is a VSSA strain. (A) Is the comparison of cytotoxicity between VS-2393 and VI-2562. (B) Is the comparison of cytotoxicity between VS-1201 and VI-1130. (C) Is the comparison of cytotoxicity among SA0534, SA0534-V8, and SA0534-V16.

Article Snippet: The clinical S. aureus strain SA0534 and laboratory-induced vancomycin resistance strains (SA0534-V8 and SA0534-V16) were obtained from Yuanyuan Dai at The Affiliated Provincial Hospital of Anhui Medical University ( ).

Techniques: Cytotoxicity Assay, Control, Positive Control, Comparison

Journal: Frontiers in Microbiology

Article Title: Comparative Analysis of Virulence and Toxin Expression of Vancomycin-Intermediate and Vancomycin-Sensitive Staphylococcus aureus Strains

doi: 10.3389/fmicb.2020.596942

Figure Lengend Snippet: The survival percentage in human blood between VISA strains and VSSA strains. The number of CFU was detected at the point at 0–3 h to calculate the rates of survival for three independent VSSA and VISA pairs exposed to human blood. Each test was repeated independently. Values are means ± SD (three repeated different experiments). The VS- indicates that this strain belongs to VSSA. The VI- indicates that the strain belongs to VISA. The SA0534-V8 and SA0534-V16 is VISA strains while SA0534 is a VSSA strain. (A) Is the comparison of survival percentage in human blood between VS-2393 and VI-2562. (B) Is the comparison of survival percentage in human blood between VS-1201 and VI-1130. (C) Is the comparison of survival percentage in human blood among SA0534, SA0534-V8, and SA0534-V16.

Article Snippet: The clinical S. aureus strain SA0534 and laboratory-induced vancomycin resistance strains (SA0534-V8 and SA0534-V16) were obtained from Yuanyuan Dai at The Affiliated Provincial Hospital of Anhui Medical University ( ).

Techniques: Comparison